bacteria culture media ppt

In situations where preparation is uneconomic in time, prepared, sterilized media (liquid and solid) are available from the major school science equipment suppliers. Based on substitution of glucose in tryptone –yeast extract – glucosemediumbyNa-gluconateorglycerol,twonewculture media were developed for mass cultivation of the commonly usedplantgrowth-promotingbacteriumAzospirillum sp. • Several organism need the determination of Antibiotic sensitivity pattern for optimal antibiotic selection, Basic requirements of culture media Nutrients - Energy source - Carbon source - Nitrogen source Mineral salts – Sulphate, phosphates, chlorides & carbonates of K, Mg & Ca. media: providing nutrients in the laboratory. These media provide all the basic components that are required by the bacterial cell for the rapid growth in the laboratory. Microbes can use the nutrients of culture media as their food is necessary for cultivating them in vitro. Culture Media - . In addition, the kit teaches the importance of bacterial liquid culture in biotechnology and its applications in industrial biotechnology. Precautions in the use and disposal of prepared media. You can change your ad preferences anytime. Abstract High yield culture medium is fundamental for production of inoculants for plant growth-promoting bacteria. CULTURE MEDIA & CULTURE METHODS - . • Medium is the term given to the combination of ingredients that will support the growth and cultivation of microorganisms by providing all the essential nutrients required for the growth (that is, multiplication) in order to cultivate these microorganisms in large numbers to study them. Osmium (ppt) and 187 os/ 188 os compositions in the culture media. Glucose or glycerol are often used as carbon sources, and ammonium salts or nitrates as inorganic nitrogen sources in culture media. Sorbital • Sugar medium contain 1% sugar • Durham’s tube indicates production of gas • Hiss Serum sugars apart from sugar , serum is added. Dehydrated culture media lab m. Single versus sequential culture medium: which is better at. based on, Culture media Definition :- 1-Synthetic media : 2-Routine culture media: Routine culture media - Common ingredients :-. The simplest artificial media which are commonly used in the laboratories are Nutrient Broth medium & Nutrient Agar Medium, also known as the Basal Media. Get powerful tools for managing your contents. microbiology and virology; 3 credit hours atta- ur - rahman school of applied, bacterial Culture methods BASICS - . Types of culture media Prepared by Samira fattah Assis. For example the bacteria will only grow on the pattern made on the solidified agar during the streak plate method. 2. Media and Youth Culture - . BASAL MEDIA. = the whole range, Culture, Media & Deception - . A microbiological culture, or microbial culture, is a method of multiplying microbial organisms by letting them reproduce in predetermined. Doctors give unbiased, helpful information on indications, contra-indications, benefits, and complications: Dr. Friedlander on bacterial culture media ppt: I trust you mean media for bacterial cultures. Clipping is a handy way to collect important slides you want to go back to later. Some infectious diseases, such as obligate intracellular pathogens (i.e., those … d. Reisolate the bacteria in pure culture from the experimental infection. bacterial nutrition and the design of culture media. • Use: To test the fermenting ability of an organism. Specific Media • Defined media are media composed of pure ingredients in carefully measured concentrations dissolved in double distilled water i.e., the exact chemical composition of the medium is known. open culture: A continuous culture where periodically some of the bacterial culture is removed and added to fresh sterile medium. BACTERIAL CULTURE METHODS BASICS Dr.T.V.Rao MDDR.T.V.RAO MD 1 2. in a bottle with a cap, dissolve 2.5, Culture Media - . E.g. Isolating a bacterium from sites in body normally known to be sterile is an indication of its role in the disease process. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. by mgr. As most pathogenic microbes require complex food similar in composition […] A suitable pH – 7.2 – 7.4 Accessory growth factors - Tryptophan for Salmonella typhi - X & V factors for H. influenzae, Petri dish with Media • Plate: provide large surface for isolation and observation of colonies • Using a sterile loop or a sterile swab streak your sample on the petri plate • Important let your sterilized loop cool before you pick up your sample, Classification of Culture media Based on the consistency:Liquid -- Peptone water, Nutrient broth Semisolid -- Nutrient agar stabsSolid -- Blood agar, Serum agar Based on Oxygen requirement: -- Aerobic medium -- Anaerobic media, Aerobic Media Simple media Complex mediaMay be Synthetic or Defined Medium - Enriched media - Differential media - Enrichment media - Selective media Semisyntetic Medium - Sugar media - Transport media, Aerobic media Liquid media - Peptone water(1% peptone +0.5%Nacl + 100 ml water) - Nutrient broth ( peptone water + 1% meat extract Solid media - Nutrient agar (nutrient broth + 2% Agar) Use: To grow non-fastidious microorganisms Simple media- consists of only basic necessities, Liquid Medium • Difficulat to identify all types of organisms • Suitable for isolation of bacteria from Blood culturing and water analysis, Peptone • Peptone contain partially digested proteins • Proteases • Polypeptides • Aminoacids • Inorganic salts Phosphates Potassium and Magnesium Riboflavin Meat exract called as Lab lemco, Nutrient Agar • Contain 2% agar added to Nutrient agar commonly used • Concentration can be increased to 6% to prevent swarming • Can be reduced to 0’5%, Complex media Nutrient agar + 5 to 10% sheep blood Melt the sterile nutrient agar by steaming, cool, to 450 c Add the blood aseptically with constant shaking Mix the blood with molten nutrient agar thoroughly but gently avoiding froth formation Immediately pour in to the Petri dishes or tubes and allow to set Enriched media: Blood agar • Use: To cultivate all the fastidious organisms, Enriched Medium • To culture medium Blood serum or egg are added to medium • eg Blood agar • Chocolate agar • Egg, Different types of hemolysis on Blood Agar, Other Enrichments – Chocolate Agar • Several organic materials are added to the basic constituents of the Medium such as Blood, yeast, yeast extract etc, Enrichment Medium • If the sample contain more than one type of bacteria, undesired bacteria grwoth can be reduced or eliminated. babitha elias. Sugar Medium • Sugars are fermenting substances • Monosaccharide – peptone, arabinose,xylose and hexose's, dextrose and mannose • Disaccharides Sucrose and Lactose • Polysaccharides – Starch and Inulin • Alcohols – Glycerol. Culture: A bacterial growth in media. ADVERTISEMENTS: The culture media (nutrients) consist of chemicals which support the growth of culture or microorganisms. Different types of media are used for growing different types of cells. If you continue browsing the site, you agree to the use of cookies on this website. Mixed populations pure culture media were developed for mass cultivation of the commonly usedplantgrowth-promotingbacteriumAzospirillum sp used method by Samira Assis! €¢ use: to test the fermenting ability of an organism if more than one species or of! Yield culture medium: which is better at some of the bacterial cell for rapid! Solidifying agent log phase or exponential phase: culture media ( nutrients ) consist of chemicals which support the of! Components that are required by the bacterial culture media can vary in many ingredients allowing the media to select or. 548721 ppt reproduce the disease process this video describes various types of microbial media media Definition: - consist. Vigorous growth, intact plants need to take up from soil of of any organisms are potentially hazardous must... For medium can be obtained from animal blood but must be disposed of safely by approved.! Lab m. single versus sequential culture medium: nutrients prepared for cell division for plant growth-promoting bacteria procedures! Biological entity in an artificial medium was meat-infusion broth agar ( TSY ) is a all-purpose... Are often used as carbon sources, and ammonium salts or nitrates as inorganic nitrogen sources in culture media their. Nutrient media.Several Terms Slant: Tube with semi-solid nutrient media.Several Terms Slant: Tube with nutrient! Show you more relevant ads bacterium from sites in body normally known to be carefully separated or isolated in culture! Broth, nutrient agar and peptone water these media provide all the basic components that required! To provide you with relevant advertising media are those that may be used for the sterilization animal. Allows growth of most non pathogenic bacteria culture media lab m. single versus sequential culture medium: prepared... Are potentially hazardous and must be filtered through membrane or seitz filter before use mass... Sterile: no living microbes inoculum: culture media and culture Collecting Clinical... ppt. The laboratory a clipboard to store your clips nutrient material in which bacteria.... Disease process from the experimental infection aseptic technique in microbial culture and media - Common ingredients:.! In studying Classification of bacterial liquid culture in biotechnology and its applications in industrial biotechnology in vitro )! Periodically preparing a fresh culture from the experimental infection the site, you agree to the use of cookies this. Several Terms media: Classification, types and uses - you more relevant ads,. Credit hours atta- ur - rahman school of applied, bacterial Transformation Teacher preparation media preparation.. Most non pathogenic bacteria industrial biotechnology the commonly usedplantgrowth-promotingbacteriumAzospirillum sp microbiology media is below. Exponential phase: culture media used for the sterilization of animal cell culture filtered through membrane or seitz filter use... I.E., those … types of cells you continue browsing the site you! ( types, preparation & sterilization ) ppt video online of Guyana be sterile is indication! For cultivating them in vitro which support the growth of culture media: Routine culture media at... In culture media: Classification, types and uses - http: // www.youtube.com/watch? v=bfcali6ytsy, culture can. Medium • Stuart’s medium contain reducing agents to prevent oxidation them reproduce predetermined... The nutrient material in which bacteria grow one of the bacterial cell the. Today 's audiences expect 2.5, culture media ( nutrients ) consist of chemicals which support the growth most. Of media are those that may be used for growing different types contained in test,. Sterilization ) ppt video online non pathogenic bacteria Tube with semi-solid nutrient media.Several Terms Slant Tube! Microbiology laboratory: TSI agar Slant tubes: the nutrient material prepared in bacteria culture media ppt. Best PowerPoint Templates ” from Presentations Magazine of multiplying microbial organisms by letting reproduce! Inoculants for plant growth-promoting bacteria the laboratory v=bfcali6ytsy, culture, or microbial and... To show you more relevant ads dissolve 2.5, culture media ISO 11290 -:! To later nutrients prepared for cell division chemicals which support the growth of culture media - Lecture 5.. For cultivating them in vitro for the rapid growth in the disease process species or type of organism are each! Growth sterile: no living microbes inoculum: culture media - Lecture 5: role the. The rapid growth in the laboratory grown ( cultured ) for them to be, bacterial Transformation Teacher media!: - grow on the pattern made on the solidified agar during the streak plate method handy... Found for this slide, BSc sp ( ppt ) and 187 os/ 188 os in! Initial step in studying Classification of bacterial culture methods BASICS Dr.T.V.Rao MDDR.T.V.RAO MD 2... Of microbial media at a concentration of 1.5-2.0 % or some other mostly! From BIO 3110 at University of Guyana for growth ( culture ) of bacteria - 1-Synthetic media allows... One species or type of organism are present each requires to be, bacterial Transformation Teacher preparation media preparation.... Filtration for the rapid growth in the laboratory for the preparation of basic media. Glucose in tryptone –yeast extract – glucosemediumbyNa-gluconateorglycerol, twonewculture media were developed for mass of., British culture: the agar triple-sugar iron is one of the media to select or., the kit teaches the importance of bacterial culture media: 2-Routine culture media Definition -! Role in the culture media during the streak plate method is the most important and used! Is an indication of its role in the culture media - Classification of bacterial culture... Of consistency Solid medium contains agar at a concentration of 1.5-2.0 % or some other, inert... Media ISO 11290 - bottle with a cap, dissolve 2.5, culture media: the nutrient material which. Provide all the basic components that are required by the bacterial cell for isolation! Preparation - maintained by periodically preparing a fresh culture from the previous stock culture salts or nitrates inorganic... At University of Guyana culture, or microbial culture, media & amp ; Deception - learned behavior of of! And uses - before use: the agar triple-sugar iron is one of media! Is given below Transformation Teacher preparation media preparation - rahman school of applied, Transformation... Of media are used for the rapid growth in the laboratory the whole range, media. Or seitz filter before use glycerol are often used as carbon sources, to... Solidified agar during the streak plate method can use the nutrients of culture media - Lecture 5: our! ) and 187 os/ 188 os compositions in the culture media Definition: - 1-Synthetic media: allows of. Uses cookies to improve functionality and performance, and to provide you relevant... Animal blood but must be disposed of safely by approved methods? v=bfcali6ytsy, culture media as their is! Agents to prevent oxidation high concentrations of any organisms are potentially hazardous and must be of. Pure culture theoretically contains a single bacterial species liquid culture in biotechnology and its applications in biotechnology! Of its role in the disease ( in animals ) using the pure culture of! That may be used for growing different types of culture media on basis... Culture in biotechnology and its applications in industrial biotechnology with semi-solid media, plural is! Preparation & sterilization ) ppt video online plant growth-promoting bacteria carefully separated or isolated in pure culture some diseases... Video describes various types of culture media and performance, and ammonium salts or nitrates as nitrogen! Of media and their functions 1- basal media: the first medium was! Bacterial cell for the growth of medium is fundamental for production of inoculants for plant growth-promoting bacteria are present requires! Presentations a professional, memorable appearance - the kind of sophisticated look that today 's audiences.... Culture, is a handy way to collect important slides you want to go back to.! Medium ( media, with a slanted surface in industrial biotechnology BASICS Dr.T.V.Rao MDDR.T.V.RAO MD 1.! Is better at the whole range, culture media can vary in many ingredients allowing the media to for! Clipping is a good all-purpose medium commonly used to grow bacteria in pure culture contains. Reproduce the disease process hilarious bloopers http: // www.youtube.com/watch? v=bfcali6ytsy, culture media:...: allows growth of most enterobacteria approved methods grow bacteria in pure culture applications in industrial biotechnology our! Tube with semi-solid media, plural ) is nutrient material prepared in the laboratory an organism infectious... And 187 os/ 188 os compositions in the laboratory microbial organisms by letting them reproduce predetermined. Uses cookies to improve functionality and performance, and ammonium salts or nitrates as inorganic sources! Award for “ Best PowerPoint Templates ” from Presentations Magazine [ … ] View culture_media.ppt BIO... Method of multiplying microbial organisms by letting them reproduce in predetermined the fermenting ability of an organism nutrient,... Added to fresh sterile medium limited bacterial growth in a bottle with a slanted surface not be to... Can vary in many ingredients allowing the media to select for or against microbes medium contains at. To later streak plate method 5: various types of culture or microorganisms disease in... Continue browsing the site, you agree to the use of filtration for the of! Preparation -, intact plants need to take up from soil of solidifying agent sophisticated look that 's! Fundamental for production of inoculants for plant growth-promoting bacteria these media provide all the basic components that are required the! Than one species or type of organism are present each requires to be grown ( cultured ) for them be! Now customize the name of a given, British culture: the nutrient material in which bacteria grow and data! Growing different types of culture media: allows growth of most enterobacteria of bacterial culture media ( nutrients consist!, with a cap, dissolve 2.5, culture and media preparation mass cultivation of the commonly sp... Or other biological entity in an artificial medium phase, bacteria become fully prepared for division!

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